2021학년도 1학기 제 2회 CNS 융합세미나 일정을 안내드립니다.
• 날짜 : 2021년 4월 1일 목요일 오후 12시
• 연사 : 의생명시스템학부 김준일 교수
• 장소 : 온라인 ZOOM 세미나(https://ssu-ac-kr.zoom.us/s/7781420554)
• 제목 : Niche-specific gene expression using PICseq analysis during mouse embryonic development
• 초록
Cells continuously interact with each other to provide signaling cues to the neighboring cells during development. Cell-cell contact is a fundamental way of communication between two interacting cells. However, our understanding of how cell-cell contact administers lineage specification remains poor.
To understand the role of cell-cell contact during embryogenesis, we used RNA sequencing from physically interacting cells (PICseq) as well as single cells (scRNAseq) from developing mouse embryos between embryonic day (E) 7.5 and E9.5. We identified a set of genes that are highly expressed when two types of cells interact with each other but not when considering cells individually. Intriguingly, cell types show gene expression profiles specific to the interacting cell type combination. For instance, Nkx2-1 is only expressed in neural progenitor (NP) cells interacting with definitive endoderm (DE) in mouse embryos at around e8.5. We further designed an approach to predict the neighboring cell type given the transcriptome of a cell. Based on our observation, we show spatial relationships of single cells in the 2-dimensional embeddings. Our results may explain the role of cell-contact for proper developmental processes.
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